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[Construction of a Mouse Model for Myeloproliferative Neoplasms and an Evaluation System].

Zhongguo shi yan xue ye xue za zhi - 1 Aug 2023

Wang Shu-Jin, Yu Xiang-Ru, Zhang Qi-Gang, Li Yan-Jie, Fu Chun-Ling, Xu Kai-Lin

Abstract excerpt

OBJECTIVE: To construct a myeloproliferative neoplasms (MPN) transplanted mouse model with JAK2-V617F, MPLW515L or CALR-Type I gene mutation, and establish a systematic evaluation system to verify the success of model construction. METHODS: The bone marrow c-kit+ cells of the mice were obtained by the following steps: The mice were killed by cervical dislocation, the femur, tibia and ilium were separated, and the...

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Identifiers

PMID: 37551485
DOI: 10.19746/j.cnki.issn.1009-2137.2023.04.028

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