M2 plants derived from different tillers of a chemically mutagenized rice M1 plant carry independent sets of mutations.

Abstract excerpt

Generation of mutant populations with high genetic diversity is key for mutant screening and crop breeding. For this purpose, the single-seed descent method, in which one mutant line is established from a single mutagenized seed, is commonly used. This method ensures the independence of the mutant lines, but the size of the mutant population is limited because it is no greater than the number of fertile M1...

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