Article
MNU-induced mutant pools and high performance TILLING enable finding of any gene mutation in rice.
Molecular genetics and genomics : MGG - 1 Mar 2008
Suzuki Tadzunu, Eiguchi Mitsugu, Kumamaru Toshihiro, Satoh Hikaru, Matsusaka Hiroaki, Moriguchi Kazuki, Nagato Yasuo, Kurata Nori
Abstract excerpt
Mutant populations are indispensable genetic resources for functional genomics in all organisms. However, suitable rice mutant populations, induced either by chemicals or irradiation still have been rarely developed to date. To produce mutant pools and to launch a search system for rice gene mutations, we developed mutant populations of Oryza sativa japonica cv. Taichung 65, by treating single zygotic cells with...
Topics
- Alleles
- Base Sequence
- DNA Mutational Analysis
- DNA Primers
- DNA, Plant
- Genes, Plant
- Genetic Techniques
- Methylnitrosourea
- Mutagenesis
- Mutagens
- Mutation