Article
An ethyl methanesulfonate-induced neutral mutant-bridging method efficiently identifies spontaneously mutated genes in rice.
The Plant journal : for cell and molecular biology - 1 Nov 2020
Hu Wei, Zhou Tianhao, Hu Gang, Wu Hong, Han Zhongmin, Xiao Jinghua, Li Xianghua, Xing Yongzhong
Abstract excerpt
Spontaneous mutants are mainly obtained from tissue culture or natural occurrences in plants. The traditional strategy for identifying spontaneously mutated genes is to continuously backcross these mutants to another variety and develop a near-isogenic F2 population for map-based cloning or bulked segregant analysis. However, this strategy is time-consuming. Here, we have developed a new method to efficiently...
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