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Article

Scanning the effects of ethyl methanesulfonate on the whole genome of Lotus japonicus using second-generation sequencing analysis.

G3 (Bethesda, Md.) - 6 Feb 2015

Mohd-Yusoff Nur Fatihah, Ruperao Pradeep, Tomoyoshi Nurain Emylia, Edwards David, Gresshoff Peter M, Biswas Bandana, Batley Jacqueline

Abstract excerpt

Genetic structure can be altered by chemical mutagenesis, which is a common method applied in molecular biology and genetics. Second-generation sequencing provides a platform to reveal base alterations occurring in the whole genome due to mutagenesis. A model legume, Lotus japonicus ecotype Miyakojima, was chemically mutated with alkylating ethyl methanesulfonate (EMS) for the scanning of DNA lesions throughout...

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Identifiers

PMID: 25660167
DOI: 10.1534/g3.114.014571
PMCID: PMC4390572

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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