Article
An efficient rice mutagenesis system based on suspension-cultured cells.
Journal of integrative plant biology - 1 Feb 2013
Chen Yuan-Ling, Liang Hui-Lin, Ma Xing-Liang, Lou Su-Lin, Xie Yong-Yao, Liu Zhen-Lan, Chen Le-Tian, Liu Yao-Guang
Abstract excerpt
Plant mutants are important bio-resources for crop breeding and gene functional studies. Conventional methods for generating mutant libraries by mutagenesis of seeds with physical or chemical agents are of low efficiency. Here, we developed a highly-efficient ethyl methanesulfonate (EMS) mutagenesis system based on suspension-cultured cells, with rice (Oryza sativa L.) as an example. We show that treatment of...
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