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Article

High-Throughput Sequencing of Phage Display Libraries Reveals Parasitic Enrichment of Indel Mutants Caused by Amplification Bias.

International journal of molecular sciences - 24 May 2021

Plessers Sander, Van Deuren Vincent, Lavigne Rob, Robben Johan

Abstract excerpt

The combination of phage display technology with high-throughput sequencing enables in-depth analysis of library diversity and selection-driven dynamics. We applied short-read sequencing of the mutagenized region on focused display libraries of two homologous nucleic acid modification eraser proteins-AlkB and FTO-biopanned against methylated DNA. This revealed enriched genotypes with small indels and concomitant...

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Identifiers

PMID: 34073702
DOI: 10.3390/ijms22115513
PMCID: PMC8197208

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