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Tuning Thermostability and Catalytic Efficiency of Aflatoxin-Degrading Enzyme by Error-prone PCR.

Applied microbiology and biotechnology - 1 Aug 2023

Jia Ru, Tian Senmiao, Yang Zhaofeng, Sadiq Faizan Ahmed, Wang Lan, Lu Simeng, Zhang Guohua, Li Jianhui

Abstract excerpt

In our previous work, a recombinant aflatoxin-degrading enzyme derived from Myxococcus fulvus (MADE) was reported. However, the low thermal stability of the enzyme had limitations for its use in industrial applications. In this study, we obtained an improved variant of recombinant MADE (rMADE) with enhanced thermostability and catalytic activity using error-prone PCR. Firstly, we constructed a mutant library...

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Identifiers

PMID: 37300712
DOI: 10.1007/s00253-023-12610-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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