Article
Engineering of a thermostable β-1,3-1,4-glucanase from Bacillus altitudinis YC-9 to improve its catalytic efficiency.
Journal of the science of food and agriculture - 15 Jan 2016
Mao Shurui, Gao Peng, Lu Zhaoxin, Lu Fengxia, Zhang Chong, Zhao Haizhen, Bie Xiaomei
Abstract excerpt
BACKGROUND: Error-prone polymerase chain reaction (PCR) is frequently used in directed evolution of enzymes to modify their quality. In this study, error-prone PCR was used to improve the catalytic efficiency of β-1,3-1,4-glucanase from Bacillus altitudinis YC-9. RESULTS: By screening, the mutant...
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