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Benchmarking microbial DNA enrichment protocols from human intestinal biopsies

26 Apr 2023

Abstract excerpt

Shotgun metagenomic sequencing is a powerful tool for studying bacterial communities in their natural habitats or sites of infection, without the need for cultivation. However, low microbial signals in metagenomic sequencing can be overwhelmed by host DNA contamination, resulting in decreased sensitivity for microbial read detection. Several commercial kits and other methods have been developed to enrich...

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PMID: 37180976
DOI: 10.3389/fgene.2023.1184473

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