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High Sensitivity of Shotgun Metagenomic Sequencing in Colon Tissue Biopsy by Host DNA Depletion

26 Sept 2022

Abstract excerpt

The high host genetic background of tissue biopsies hinders the application of shotgun metagenomic sequencing in characterizing the tissue microbiota. We proposed an optimized method that removed host DNA from colon biopsies and examined the effect on metagenomic analysis. Human or mouse colon biopsies were divided into two groups, with one group undergoing host DNA depletion and the other serving as the control....

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PMID: 36174929
DOI: 10.1016/j.gpb.2022.09.003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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