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DNA extraction of microbial DNA directly from infected tissue: an optimized protocol for use in nanopore sequencing

19 Feb 2020

Abstract excerpt

Identification of bacteria causing tissue infections can be comprehensive and, in the cases of non- or slow-growing bacteria, near impossible with conventional methods. Performing shotgun metagenomic sequencing on bacterial DNA extracted directly from the infected tissue may improve time to diagnosis and targeted treatment considerably. However, infected tissue consists mainly of human DNA (hDNA) which hampers...

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Share to Bacterial Identification and Susceptibility Testing
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PMID: 32076089
DOI: 10.1038/s41598-020-59957-6

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