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Comparison of DNA extraction methods for 16S rRNA gene sequencing in the analysis of the human gut microbiome

24 Jun 2023

Abstract excerpt

The gut microbiome is widely analyzed using high-throughput sequencing, such as 16S rRNA gene amplicon sequencing and shotgun metagenomic sequencing (SMS). DNA extraction is known to have a large impact on the metagenomic analyses. The aim of this study was to compare DNA extraction protocols for 16S sequencing. In that context, four commonly used DNA extraction methods were compared for the analysis of the gut...

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PMID: 37355726
DOI: 10.1038/s41598-023-33959-6

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