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Article

Improving saliva shotgun metagenomics by chemical host DNA depletion

26 Feb 2018

Abstract excerpt

BACKGROUND: Shotgun sequencing of microbial communities provides in-depth knowledge of the microbiome by cataloging bacterial, fungal, and viral gene content within a sample, providing an advantage over amplicon sequencing approaches that assess taxonomy but not function and are taxonomically limited. However, mammalian DNA can dominate host-derived samples, obscuring changes in microbial populations because few...

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PMID: 29482639
DOI: 10.1186/s40168-018-0426-3

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