Article
A unique and reliable fecal DNA extraction method for 16S rRNA gene and shotgun metagenomic sequencing in the analysis of the human gut microbiome
2020-08-06
Abstract excerpt
<title>Abstract</title> <p><bold>Background: </bold>The gut microbiome is widely analyzed using high-throughput sequencing, such as 16S rRNA gene amplicon sequencing and shotgun metagenomic sequencing (SMS). DNA extraction is known to have a large impact on the metagenomic analyses. The aim of this study was to select a unique and best performing DNA extraction protocol for both metagenomic sequencing methods. In...
Topics
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- Clostridium difficile and Clostridium perfringens research
- Colorectal Cancer Screening and Detection
- Dysphagia Assessment and Management
- Enterobacteriaceae and Cronobacter Research
- Epigenetics and DNA Methylation
- Fecal contamination and water quality
- Gut microbiota and health
- Mycobacterium research and diagnosis
Identifiers and source
- Literature Corpus work
- 3628e9fa-57a1-5504-b501-0951beeec491
- DOI
- 10.21203/rs.3.rs-52279/v1
