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Microbial DNA extraction of high-host content and low biomass samples: Optimized protocol for nasopharynx metagenomic studies

21 Dec 2022

Abstract excerpt

Introduction: Low microbial biomass and high human DNA content in nasopharyngeal aspirate samples hinder comprehensive characterization of microbiota and resistome. We obtained samples from premature infants, a group with increased risk of developing respiratory disorders and infections, and consequently frequent exposure to antibiotics. Our aim was to devise an optimal protocol for handling nasopharyngeal...

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PMID: 36620054
DOI: 10.3389/fmicb.2022.1038120

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