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DNA methylation protocol for analyzing cell-free DNA in the spent culture medium of human preimplantation embryos

21 Apr 2023

Abstract excerpt

Cell-free DNA (cfDNA) in spent embryo culture media (SECM) provides prospects for noninvasive preimplantation genetic testing. Here, we present a post-bisulfite-adapter-tagging (PBAT)-based whole-genome DNA methylation sequencing protocol (SECM-PBAT) for human SECM cfDNA analysis. We describe steps for SECM lysis, bisulfite conversion and purification, preamplification by random priming, tagging adapter II, and...

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PMID: 37086412
DOI: 10.1016/j.xpro.2023.102247

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