Article
Primerdiffer: a python command-line module for large-scale primer design in haplotype genotyping.
Bioinformatics (Oxford, England) - 3 Apr 2023
Ren Xiaoliang, Shao Yanwen, Zhang Yiwen, Ni Ying, Bi Yu, Li Runsheng
Abstract excerpt
MOTIVATION: Primer design is a routine practice for modern molecular biology labs. Bioinformatics tools like primer3 and primer-blast have standardized the primer design for a specific region. However, large-scale primer design, especially for genome-wide screening, is still a labor-intensive job for most wet-lab researchers using these pipelines. RESULTS: Here, we present the primerdiffer pipeline, which can be...
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