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Article

Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction.

BMC bioinformatics - 18 Jun 2012

Ye Jian, Coulouris George, Zaretskaya Irena, Cutcutache Ioana, Rozen Steve, Madden Thomas L

Abstract excerpt

BACKGROUND: Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in certain orientations and within certain distances that allow undesired amplification. The process of designing specific primers typically involves two stages. First, the...

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Identifiers

PMID: 22708584
DOI: 10.1186/1471-2105-13-134
PMCID: PMC3412702

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Source receipt: pubmed, retrieved 7 Sept 2026.

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