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Helping decision making for reliable and cost-effective 2b-RAD sequencing and genotyping analyses in non-model species.

Molecular ecology resources - 1 May 2020

Barbanti Anna, Torrado Hector, Macpherson Enrique, Bargelloni Luca, Franch Rafaella, Carreras Carlos, Pascual Marta

Abstract excerpt

High-throughput sequencing has revolutionized population and conservation genetics. RAD sequencing methods, such as 2b-RAD, can be used on species lacking a reference genome. However, transferring protocols across taxa can potentially lead to poor results. We tested two different IIB enzymes (AlfI and CspCI) on two species with different genome sizes (the loggerhead turtle Caretta caretta and the sharpsnout...

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PMID: 32061018
DOI: 10.1111/1755-0998.13144

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