Article
GETPrime 2.0: gene- and transcript-specific qPCR primers for 13 species including polymorphisms.
Nucleic acids research - 4 Jan 2017
David Fabrice P A, Rougemont Jacques, Deplancke Bart
Abstract excerpt
GETPrime (http://bbcftools.epfl.ch/getprime) is a database with a web frontend providing gene- and transcript-specific, pre-computed qPCR primer pairs. The primers have been optimized for genome-wide specificity and for allowing the selective amplification of one or several splice variants of most known genes. To ease selection, primers have also been ranked according to defined criteria such as genome-wide...
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