Article
The Significance of PCR Primer Design in Genetic Diversity Studies: Exemplified by Recent Research into the Genetic Structure of Marine Species.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022
Delghandi Madjid, Delghandi Marit Pedersen, Goddard Stephen
Abstract excerpt
Genetic markers are widely applied in the study of genetic diversity for many species. The approach incorporates a Polymerase Chain Reaction (PCR) amplification of targeted sequences in the genome. Crucial for the overall success of a PCR experiment is the careful design of synthetic oligonucleotide primers. Ideally designed primer pairs will ensure the efficiency and specificity of the amplification reaction,...
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