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Deep Mutational Scanning of an Oxygen-Independent Fluorescent Protein CreiLOV for Comprehensive Profiling of Mutational and Epistatic Effects.

ACS synthetic biology - 19 May 2023

Chen Yongcan, Hu Ruyun, Li Keyi, Zhang Yating, Fu Lihao, Zhang Jianzhi, Si Tong

Abstract excerpt

Oxygen-independent, flavin mononucleotide-based fluorescent proteins (FbFPs) are promising alternatives to green fluorescent protein in anaerobic contexts. Deep mutational scanning performs systematic profiling of protein sequence-function relationships but has not been applied to FbFPs. Focusing on CreiLOV from Chlamydomonas reinhardtii, we created and analyzed two comprehensive mutant collections: (1)...

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PMID: 37066862
DOI: 10.1021/acssynbio.2c00662
PMCID: PMC10204710

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