Article
Diagnostic value of pyrosequencing for the BRAF V600E mutation in ultrasound-guided fine-needle aspiration biopsy samples of thyroid incidentalomas.
Clinical endocrinology - 1 Jan 2009
Jo Young Suk, Huang Songmei, Kim Yun-Jeung, Lee Ihn-Suk, Kim Song-Soo, Kim Je-Ryong, Oh Taejeong, Moon Youngho, An Sungwhan, Ro Heung-kyu, Kim Jin-Man, Shong Minho
Abstract excerpt
CONTEXT: Dideoxy sequencing is the most commonly used method for detecting the BRAF(V600E) mutation in thyroid cancer and melanoma. However, this gold standard method often makes less definite results in detecting the BRAF(V600E) mutation when there are relatively low amounts of the mutant template in biopsy specimens, which are invariably contaminated with normal tissues. Pyrosequencing, which measures the...
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