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Introduction of Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.).

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Quach Truyen, Nguyen Hanh, Meyer Olivia, Sato Shirley J, Clemente Tom Elmo, Guo Ming

Abstract excerpt

Cas9-based genome editing is a powerful genetic tool for loci specifically targeted for genome modification. This chapter describes up-to-date protocols using Cas9-based genome editing technology, including vector construction with GoldenBraid assembly, Agrobacterium-mediated soybean transformation, and identification of editing in the genome.

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PMID: 36995632
DOI: 10.1007/978-1-0716-3131-7_17

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