Article
High-Throughput CRISPR/Cas9 Mutagenesis Streamlines Trait Gene Identification in Maize.
The Plant cell - 1 May 2020
Liu Hai-Jun, Jian Liumei, Xu Jieting, Zhang Qinghua, Zhang Maolin, Jin Minliang, Peng Yong, Yan Jiali, Han Baozhu, Liu Jie, Gao Fan, Liu Xiangguo, Huang Lei, Wei Wenjie, Ding Yunxiu, Yang Xiaofeng, Li Zhenxian, Zhang Mingliang, Sun Jiamin, Bai Minji, Song Wenhao, Chen Hanmo, Sun Xi'ang, Li Wenqiang, Lu Yuming, Liu Ya, Zhao Jiuran, Qian Yangwen, Jackson David, Fernie Alisdair R, Yan Jianbing
Abstract excerpt
Maize (Zea mays) is one of the most important crops in the world. However, few agronomically important maize genes have been cloned and used for trait improvement, due to its complex genome and genetic architecture. Here, we integrated multiplexed CRISPR/Cas9-based high-throughput targeted mutagenesis with genetic mapping and genomic approaches to successfully target 743 candidate genes corresponding to traits...
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