Article
Integration, abundance, and transmission of mutations and transgenes in a series of CRISPR/Cas9 soybean lines.
BMC biotechnology - 24 Feb 2020
Michno Jean-Michel, Virdi Kamaldeep, Stec Adrian O, Liu Junqi, Wang Xiaobo, Xiong Yer, Stupar Robert M
Abstract excerpt
BACKGROUND: As with many plant species, current genome editing strategies in soybean are initiated by stably transforming a gene that encodes an engineered nuclease into the genome. Expression of the transgene results in a double-stranded break and repair at the targeted locus, oftentimes resulting in mutation(s) at the intended site. As soybean is a self-pollinating species with 20 chromosome pairs, the...
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