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A DNA-free and genotype-independent CRISPR/Cas9 system in soybean.

Plant physiology - 2 Dec 2024

Kuwabara Chikako, Miki Ryuji, Maruyama Nobuyuki, Yasui Masanori, Hamada Haruyasu, Nagira Yozo, Hirayama Yumiko, Ackley Wataru, Li Feng, Imai Ryozo, Taoka Naoaki, Yamada Tetsuya

Abstract excerpt

Here, we report a smart genome editing system for soybean (Glycine max) using the in planta bombardment-ribonucleoprotein (iPB-RNP) method without introducing foreign DNA or requiring traditional tissue culture processes such as embryogenesis and organogenesis. Shoot apical meristem (SAM) of embryonic axes was used as the target tissue for genome editing because the SAM in soybean mature seeds has stem cells and...

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CRISPR-Cas Systems
Gene Editing
Genome, Plant
Genotype
Glycine max

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plants, Genetically Modified

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PMID: 39307540
DOI: 10.1093/plphys/kiae491

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

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