Article
Site-directed mutagenesis by biolistic transformation efficiently generates inheritable mutations in a targeted locus in soybean somatic embryos and transgene-free descendants in the T1 generation.
Transgenic research - 1 Feb 2021
Adachi Kohei, Hirose Aya, Kanazashi Yuhei, Hibara Miki, Hirata Toshiyuki, Mikami Masafumi, Endo Masaki, Hirose Sakiko, Maruyama Nobuyuki, Ishimoto Masao, Abe Jun, Yamada Tetsuya
Abstract excerpt
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated endonuclease 9 (Cas9) system is being rapidly developed for mutagenesis in higher plants. Ideally, foreign DNA introduced by this system is removed in the breeding of edible crops and vegetables. Here, we report an efficient generation of Cas9-free mutants lacking an allergenic gene, Gly m Bd 30K, using biolistic...
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