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An ultrasensitive method for detecting mutations from short and rare cell-free DNA.

Biosensors & bioelectronics - 15 Oct 2023

Wang Lin, Zhuang Yu, Yu Yue, Guo Zhiwei, Guo Qiaomei, Qiao Lihua, Wang Xueqing, Liang Xiaohui, Zhang Pengpeng, Li Qifan, Huang Chenjun, Cong Rong, Li Yinghui, Che Bin, Xiong Huihui, Lin Guomin, Rao Mingming, Hu Rongjun, Wang Wei, Yang Guohua, Lou Jiatao

Abstract excerpt

Circulating tumor DNA (ctDNA) was short and rare, making the detection performance of the current targeted sequencing methods unsatisfying. We developed the One-PrimER Amplification (OPERA) system and examined its performance in detecting mutations of low variant allelic frequency (VAF) in various samples with short-sized DNA fragments. In cell line-derived samples containing sonication-sheared DNA fragments with...

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PMID: 37542979
DOI: 10.1016/j.bios.2023.115548

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