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Circulating Tumor DNA Monitoring in Patients with Uveal Melanoma Using Mutation-Agnostic Multiplex Drop-Off ddPCR Assays.

Analytical chemistry - 19 May 2026

Rampanou Aurore, Rodrigues Manuel, Bidard François-Clément, Cassoux Nathalie, Matet Alexandre, Vincent-Salomon Anne, Jeannot Emmanuelle, Bieche Ivan, Roman-Roman Sergio, Stern Marc-Henri, Renault Shufang

Abstract excerpt

To address the limitations of existing approaches─targeted next-generation sequencing (NGS) and simplex droplet digital PCR (ddPCR) assays─for circulating tumor DNA (ctDNA) detection in uveal melanoma (UM), we developed and validated mutation-agnostic multiplex drop-off ddPCR assays at the targeted loci as a novel strategy. Two multiplex drop-off ddPCR assays covering hotspot mutations in GNAQ p.Q209, GNA11...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 42082165
DOI: 10.1021/acs.analchem.6c00476
PMCID: PMC13191726

Genes

CYSLTR2
GNA11
GNAQ
MAF
PLCB4
SF3B1

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

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