Article
Quantification of rare somatic single nucleotide variants by droplet digital PCR using SuperSelective primers.
Scientific reports - 3 Nov 2023
Pablo-Fontecha Verónica, Hernández-Illán Eva, Reparaz Andrea, Asensio Elena, Morata Jordi, Tonda Raúl, Lahoz Sara, Parra Carolina, Lozano Juan José, García-Heredia Anabel, Martínez-Roca Alejandro, Beltran Sergi, Balaguer Francesc, Jover Rodrigo, Castells Antoni, Trullàs Ramon, Podlesniy Petar, Camps Jordi
Abstract excerpt
Somatic single-nucleotide variants (SNVs) occur every time a cell divides, appearing even in healthy tissues at low frequencies. These mutations may accumulate as neutral variants during aging, or eventually, promote the development of neoplasia. Here, we present the SP-ddPCR, a droplet digital PCR (ddPCR) based approach that utilizes customized SuperSelective primers aiming at quantifying the proportion of rare...
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