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Article

The application of adenine deaminase in antibody affinity maturation.

Applied microbiology and biotechnology - 1 Apr 2023

Qu Baole, Zhao Yun, An Lili, Hang Haiying

Abstract excerpt

Previously, we established a platform for antibody/protein affinity maturation based on CHO cell display. The gene of interest was mutated by activation-induced cytidine deaminase (AID), and then, a mutation library mainly containing G/C to A/T conversion was obtained by simply proliferating cells. However, the AID-induced G/C to A/T conversion limits the diversity space of the mutation library. In contrast to...

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PMID: 36929186
DOI: 10.1007/s00253-022-12324-z

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