Article
Coupling mammalian cell surface display with somatic hypermutation for the discovery and maturation of human antibodies.
Proceedings of the National Academy of Sciences of the United States of America - 20 Dec 2011
Bowers Peter M, Horlick Robert A, Neben Tamlyn Y, Toobian Rachelle M, Tomlinson Geoffrey L, Dalton Jennifer L, Jones Heather A, Chen Andy, Altobell Laurence, Zhang Xue, Macomber John L, Krapf Irina P, Wu Betty F, McConnell Audrey, Chau Betty, Holland Trevin, Berkebile Ashley D, Neben Steven S, Boyle William J, King David J
Abstract excerpt
A novel approach has been developed for the isolation and maturation of human antibodies that replicates key features of the adaptive immune system by coupling in vitro somatic hypermutation (SHM) with mammalian cell display. SHM is dependent on the action of the B cell specific enzyme, activation-induced cytidine deaminase (AID), and can be replicated in non-B cells through expression of recombinant AID. A...
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