Article
Affinity maturation of anti-TNF-alpha scFv with somatic hypermutation in non-B cells.
Protein & cell - 1 Jun 2012
Chen Shaopeng, Qiu Junkang, Chen Chuan, Liu Chunchun, Liu Yuheng, An Lili, Jia Junying, Tang Jie, Wu Lijun, Hang Haiying
Abstract excerpt
Activation-induced cytidine deaminase (AID) is required for the generation of antibody diversity through initiating both somatic hypermutation (SHM) and class switch recombination. A few research groups have successfully used the feature of AID for generating mutant libraries in directed evolution of target proteins in B cells in vitro. B cells, cultured in suspension, are not convenient for transfection and...
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