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Article

Coupling recombinase-mediated cassette exchange with somatic hypermutation for antibody affinity maturation in CHO cells.

Biotechnology and bioengineering - 1 Jan 2016

Chen Chuan, Li Nan, Zhao Yun, Hang Haiying

Abstract excerpt

Heterologous expression of activation-induced cytidine deaminase (AID) can induce somatic hypermutation (SHM) for genes of interest in various cells, and several research groups (including ours) have successfully improved antibody affinity in mammalian or chicken cells using AID-induced SHM. These affinity maturation systems are time-consuming and inefficient. In this study, we developed an antibody affinity...

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Identifiers

PMID: 26235363
DOI: 10.1002/bit.25541

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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