Frequency matters: comparison of drug resistance mutation detection by Sanger and next-generation sequencing in HIV-1.

doi:10.1093/jac/dkac430

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Article

Frequency matters: comparison of drug resistance mutation detection by Sanger and next-generation sequencing in HIV-1.

The Journal of antimicrobial chemotherapy - 2 Mar 2023

Balakrishna Suraj, Loosli Tom, Zaheri Maryam, Frischknecht Paul, Huber Michael, Kusejko Katharina, Yerly Sabine, Leuzinger Karoline, Perreau Matthieu, Ramette Alban, Wymant Chris, Fraser Christophe, Kellam Paul, Gall Astrid, Hirsch Hans H, Stoeckle Marcel, Rauch Andri, Cavassini Matthias, Bernasconi Enos, Notter Julia, Calmy Alexandra, Günthard Huldrych F, Metzner Karin J, Kouyos Roger D

Abstract excerpt

BACKGROUND: Next-generation sequencing (NGS) is gradually replacing Sanger sequencing (SS) as the primary method for HIV genotypic resistance testing. However, there are limited systematic data on comparability of these methods in a clinical setting for the presence of low-abundance drug resistance mutations (DRMs) and their dependency on the variant-calling thresholds. METHODS: To compare the HIV-DRMs detected...

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PMID: 36738248
DOI: 10.1093/jac/dkac430

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