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Article

A deep mutational scanning platform to characterize the fitness landscape of anti-CRISPR proteins.

Nucleic acids research - 11 Dec 2024

Stadelmann Tobias, Heid Daniel, Jendrusch Michael, Mathony Jan, Aschenbrenner Sabine, Rosset Stéphane, Correia Bruno E, Niopek Dominik

Abstract excerpt

Deep mutational scanning is a powerful method for exploring the mutational fitness landscape of proteins. Its adaptation to anti-CRISPR proteins, which are natural CRISPR-Cas inhibitors and key players in the co-evolution of microbes and phages, facilitates their characterization and optimization. Here, we developed a robust anti-CRISPR deep mutational scanning pipeline in Escherichia coli that combines synthetic...

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Share to Clustered Regularly Interspaced Short Palindromic Repeats
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 39558174
DOI: 10.1093/nar/gkae1052
PMCID: PMC11662660

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