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An Improved Real-Time Viability PCR Assay to Detect Salmonella in a Culture-Independent Era

25 Nov 2022

Abstract excerpt

Viability PCR (vPCR) uses a DNA intercalating dye to irreversibly bind double-stranded DNA from organisms with compromised cell membranes. This allows the selective amplification of DNA from intact cells. An optimized vPCR protocol should minimize false positives (DNA from compromised cells not fully removed) and false negatives (live cell DNA bound by the dye). We aimed to optimize a vPCR protocol using PMAxx™...

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PMID: 36499040
DOI: 10.3390/ijms232314708

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