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Improving sensitivity of single tube nested PCR to detect fastidious microorganisms

1 Jan 2020

Abstract excerpt

Single Tube Nested PCR (ST-nPCR) is of value to clinical laboratories with limited settings for the detection of fastidious microorganisms. The detection sensitivity of ST-nPCR is dependent on ensuring minimal leftovers of outer primers during the second round of the reaction. In this work, we investigated various approaches to optimize the performance of outer primers, including decreasing outer primer...

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PMID: 32042975
DOI: 10.1016/j.heliyon.2020.e03246

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