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Challenges for estimating human norovirus infectivity by viability RT-qPCR as compared to replication in human intestinal enteroids

24 Nov 2023

Abstract excerpt

Viability RT-qPCR, a molecular detection method combining viability marker pre-treatment with RT-qPCR, has been proposed to infer infectivity of viruses which is particularly relevant for non-culturable viruses or sophisticated cell culture systems. Being human noroviruses (HuNoV) most frequently associated with foodborne outbreaks, this study compared different viability techniques and infectivity in human...

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PMID: 38043474
DOI: 10.1016/j.ijfoodmicro.2023.110507

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