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Evaluation of PMA‐qPCR methodology to detect and quantify viable Shiga toxin‐producing Escherichia coli in beef burgers

2021-04-01

Abstract excerpt

Propidium monoazide (PMA) is a selective nucleic acid intercalating dye that can be combined with real‐time PCR (qPCR) in order to evaluate cell viability in food samples. The aim of the present work was to evaluate PMA‐qPCR to detect and quantify viable STEC cells in beef burgers using stx₂ as target gene. First, it was determined that 100 µM of PMA could inhibit qPCR signal from non‐viable cells and had no influ...

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Literature Corpus work
ef9a28f0-746c-5814-8c45-86219b2d609b
DOI
10.1111/jfpp.15338
Open publication

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Evaluation of PMA‐qPCR methodology to detect and quantify viable Shiga toxin‐producing Escherichia coli in beef burgersDOI 10.1111/jfpp.15338
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