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Article

Quality metrics for enhanced performance of an NGS panel using single-vial amplification technology.

Journal of clinical pathology - 14 Dec 2023

Barua Subit, Hsiao Susan, Clancy Emily, Freeman Christopher, Mansukhani Mahesh, Fernandes Helen

Abstract excerpt

AIMS: Targeted next-generation sequencing (NGS) panels, which identify genomic alterations, are the stronghold of molecular oncology laboratories. In spite of technological advances, the quantity and quality of DNA from formalin-fixed paraffin-embedded tissue and paucicellular specimens are barriers to successful sequencing. Here, we describe an NGS assay employing single tube stem-loop inhibition mediated...

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Identifiers

PMID: 36376073
DOI: 10.1136/jcp-2022-208536

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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