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Rapid fluoroquinolone resistance detection in Pseudomonas aeruginosa using mismatch amplification mutation assay-based real-time PCR.

Journal of medical microbiology - 1 Oct 2022

Madden Danielle E, McCarthy Kate L, Bell Scott C, Olagoke Olusola, Baird Timothy, Neill Jane, Ramsay Kay A, Kidd Timothy J, Stewart Adam G, Subedi Shradha, Choong Keat, Fraser Tamieka A, Sarovich Derek S, Price Erin P

Abstract excerpt

Background. Antimicrobial resistance (AMR) is an ever-increasing global health concern. One crucial facet in tackling the AMR epidemic is earlier and more accurate AMR diagnosis, particularly in the dangerous and highly multi-drug-resistant ESKAPE pathogen, Pseudomonas aeruginosa.Objectives. We aimed to develop two SYBR Green-based mismatch amplification mutation assays (SYBR-MAMAs) targeting GyrA T83I (gyrA248)...

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PMID: 36301593
DOI: 10.1099/jmm.0.001593

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