Article
Molecular analysis of ciprofloxacin resistance mechanisms in Malaysian ESBL-producing Klebsiella pneumoniae isolates and development of mismatch amplification mutation assays (MAMA) for rapid detection of gyrA and parC mutations.
BioMed research international - 1 Jan 2014
Al-Marzooq Farah, Mohd Yusof Mohd Yasim, Tay Sun Tee
Abstract excerpt
Ninety-three Malaysian extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolates were investigated for ciprofloxacin resistance. Two mismatch amplification mutation (MAMA) assays were developed and used to facilitate rapid detection of gyrA and parC mutations. The isolates were also screened for plasmid-mediated quinolone resistance (PMQR) genes including aac(6')-Ib-cr, qepA, and qnr....
Topics
- Anti-Bacterial Agents
- Base Pair Mismatch
- Base Sequence
- Cell Survival
- Ciprofloxacin
- DNA Gyrase
- DNA Mutational Analysis
- DNA Topoisomerase IV
- Klebsiella pneumoniae
- Malaysia