Article
Comparison of Mismatch Amplification Mutation Assay PCR and PCR-Restriction Fragment Length Polymorphism for Detection of Major Mutations in gyrA and parC of Escherichia coli Associated with Fluoroquinolone Resistance.
Microbial drug resistance (Larchmont, N.Y.) - 1 Jan 2000
Jazeela Kadeeja, Chakraborty Gunimala, Shetty Shruthi Seetharam, Rohit Anusha, Karunasagar Indrani, Vijaya Kumar Deekshit
Abstract excerpt
Fluoroquinolones are the drug of choice for most of the infections caused by Escherichia coli, and their indiscriminate use has resulted in increased selective pressure for antibiotic resistance. At present, sequencing is the only reliable and direct technique to detect mutations in the quinolone...
Topics
- Anti-Bacterial Agents
- DNA Gyrase
- DNA Topoisomerase IV
- Drug Resistance, Bacterial
- Escherichia coli
- Fluoroquinolones
- Humans
- Microbial Sensitivity Tests
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Quinolones