High-throughput mutagenesis identifies mutations and RNA-binding proteins controlling CD19 splicing and CART-19 therapy resistance.

doi:10.1038/s41467-022-31818-y

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Article

High-throughput mutagenesis identifies mutations and RNA-binding proteins controlling CD19 splicing and CART-19 therapy resistance.

Nature communications - 22 Sept 2022

Cortés-López Mariela, Schulz Laura, Enculescu Mihaela, Paret Claudia, Spiekermann Bea, Quesnel-Vallières Mathieu, Torres-Diz Manuel, Unic Sebastian, Busch Anke, Orekhova Anna, Kuban Monika, Mesitov Mikhail, Mulorz Miriam M, Shraim Rawan, Kielisch Fridolin, Faber Jörg, Barash Yoseph, Thomas-Tikhonenko Andrei, Zarnack Kathi, Legewie Stefan, König Julian

Abstract excerpt

Following CART-19 immunotherapy for B-cell acute lymphoblastic leukaemia (B-ALL), many patients relapse due to loss of the cognate CD19 epitope. Since epitope loss can be caused by aberrant CD19 exon 2 processing, we herein investigate the regulatory code that controls CD19 splicing. We combine high-throughput mutagenesis with mathematical modelling to quantitatively disentangle the effects of all mutations in...

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Identifiers

PMID: 36138008
DOI: 10.1038/s41467-022-31818-y
PMCID: PMC9500061

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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