Article
Implementation of an In-House Platform for Rapid Screening of SARS-CoV-2 Genome Variations.
Archives of Iranian medicine - 1 Feb 2023
Zare Ashrafi Farzane, Mohseni Marzieh, Beheshtian Maryam, Fattahi Zohreh, Ghodratpour Fatemeh, Keshavarzi Fatemeh, Behravan Hanieh, Kalhor Marzieh, Jalalvand Khadijeh, Azad Maryam, Koshki Mahdieh, Jafarpour Ali, Ghaziasadi Azam, Abdollahi Alireza, Kiani Seyed Jalal, Ataei-Pirkooh Angila, Rezaei Azhar Iman, Bokharaei-Salim Farah, Haghshenas Mohammad Reza, Babamahmoodi Farhang, Mokhames Zakiye, Soleimani Alireza, Ziaee Masood, Javanmard Davod, Ghafari Shokouh, Ezani Akram, Ansari Moghaddam Alireza, Shahraki-Sanavi Fariba, Hashemi Shahri Seyed Mohammad, Azaran Azarakhsh, Yousefi Farid, Moattari Afagh, Moghadami Mohsen, Fakhim Hamed, Ataei Behrooz, Nasri Elahe, Poortahmasebi Vahdat, Varshochi Mojtaba, Mojtahedi Ali, Jalilian Farid, Khazeni Mohammad, Moradi Abdolvahab, Tabarraei Alijan, Piroozmand Ahmad, Yahyapour Yousef, Bayani Masoumeh, Aboofazeli Amir, Ghafari Parsa, Keramat Fariba, Tavakoli Mahsa, Jalali Tahmineh, Pouriayevali Mohammad Hassan, Salehi-Vaziri Mostafa, Khorram Khorshid Hamid Reza, Najafipour Reza, Malekzadeh Reza, Kahrizi Kimia, Jazayeri Seyed Mohammad, Najmabadi Hossein
Abstract excerpt
BACKGROUND: Global real-time monitoring of SARS-CoV-2 variants is crucial to controlling the COVID-19 outbreak. The purpose of this study was to set up a Sanger-based platform for massive SARS-CoV-2 variant tracking in laboratories in low-resource settings. METHODS: We used nested RT-PCR assay, Sanger sequencing and lineage assignment for 930-bp of the SARS-CoV-2 spike gene, which harbors specific variants of...
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