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Article

Cloning and structural basis of fluorescent protein color variants from identical species of sea anemone, Diadumene lineata.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology - 1 Jul 2023

Horiuchi Yuki, Makabe Koki, Laskaratou Danai, Hatori Kuniyuki, Sliwa Michel, Mizuno Hideaki, Hotta Jun-Ichi

Abstract excerpt

Diadumene lineata is a colorful sea anemone with orange stripe tissue of the body column and plain tentacles with red lines. We subjected Diadumene lineata to expression cloning and obtained genes encoding orange (OFP: DiLiFP561) and red fluorescent proteins (RFPs: DiLiFP570 and DiLiFP571). These proteins formed obligatory tetramers. All three proteins showed bright fluorescence with the brightness of...

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PMID: 36943649
DOI: 10.1007/s43630-023-00399-0

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