Article
Use of fully modified 2'-O-methyl antisense oligos for loss-of-function studies in vertebrate embryos.
Genesis (New York, N.Y. : 2000) - 1 Mar 2011
Schneider Patricia N, Olthoff John T, Matthews Abby J, Houston Douglas W
Abstract excerpt
Antisense oligonucleotides are commonly employed to study the roles of genes in development. Although morpholino phosphorodiamidate oligonucleotides (morpholinos) are widely used to block translation or splicing of target gene products' the usefulness of other modifications in mediating RNase-H independent inhibition of gene activity in embryos has not been investigated. In this study, we investigated the extent...
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